Holders Asante Kotoko won a penalty shoot-out to beat third-tier Storm Academy on Sunday and sneak into the MTN FA Cup last 32.
Both teams failed to find the back of the net in regulation time.
The Porcupine Warriors recovered from Wahab Adams’ opening miss to scale the hurdle.
Their rivals, Hearts of Oak needed a Joseph Esso lone strike to progress at the expense of Accra Young Wise.
In the all premier league clash, AshantiGold defeated Bechem United in Obuasi.
League leaders Medeama overcame lower tier side OilCity FC in Sekondi.
Meanwhile, Berekum Chelsea and Ebusua Dwarfs were sent crashing out of the tournament by teams in the lower tier.
Mighty Jets 0-1 Tema Youth
Jeffisi United 0-2 Wa All Stars FC
Vision FC 0-1 Liberty Professionals
Dreams FC 4-1 Mepom Vatens FC
Inter Allies 1-0 Immigration FC
Emmanuel FC 0-1 Nania
AshantiGold 1-0 Bechem United
Samartex 0-0 Kerala (Karela win 4-2 on Penalties)
Akatsi All Stars 1-0 Agbozume Weavers
Asante Kotoko 0-0 Storm Academy (Kotoko win 6-5 on penalties)
Bis Paradise 1-1 Nzema Kotoko (Paradies win 5-4 on penalties)
Unistar Academy 2-0 Swanpomaa F C
Oil City 1-2 Medeama SC
Accra Young Wise 0-1 Hearts of Oak
Aduana Stars 1-0 Young Wise Apostles
Eleven Wonders 3-1 Techiman City FC
Likpe Heroes 0-0 WAFA (WAFA win 5-3 on penalties)
New Edubiase 1-0 Thunderbolt FC
Sankara Nationals FC 0-8 Wa Suntaa FC
Bebeto FC 0-0 Hearts of Lions (Bebeto win 4-3 on penalties)
Soccer Intellectuals FC 0-4 Elmina Sharks
Bolga Soccer Masters 3-0 Zuarungu FC
River Plate Athletic Club 0-1 Asokwa Deportivo
Kotoku Royals vs Madina Republicans – Rained off
Ebusua Dwarfs 0-1 Star Madrid FC
Berekum Chelsea 0-1 Berekum Arsenal
Bibiani Gold Stars 5-2 Enchi All Stars
Kintampo Top Talent 4-1 Unity FC
Kenyasi New Dreams vs Bamboi City FC – Rained off
Techiman Eleven Wonders 3-1 Techiman City FC
Winneba United FC 2-0 Venomous Vipers
DC United 0-0 Brong Ahafo United (DC United win 5-4 on penalties)